Gene description for ompA
Gene name outer membrane protein A
Gene symbol ompA
Other names/aliases ECK0948
con
tolG
tut
Species Escherichia coli str. K-12 substr. MG1655
 Experiment description of studies that identified ompA in Escherichia coli

1
Experiment ID373
Identified moleculeprotein
Extracellular vesicle typeMembrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 17787032   
OrganismEscherichia coli
Experiment descriptionGlobal proteomic profiling of native outer membrane vesicles derived from Escherichia coli.
AuthorsLee EY, Bang JY, Park GW, Choi DS, Kang JS, Kim HJ, Park KS, Lee JO, Kim YK, Kwon KH, Kim KP, Gho YS.
Journal name Proteomics
Publication year2007
SampleEscherichia coli
Sample nameNormal-Escherichia coli DH5α
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
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2
Experiment ID2184
Identified moleculeprotein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34276573   
OrganismEscherichia coli
Experiment descriptionDifferential Packaging Into Outer Membrane Vesicles Upon Oxidative Stress Reveals a General Mechanism for Cargo Selectivity
AuthorsOrench-Rivera N, Kuehn MJ.
Journal name Front Microbiol
Publication year2021
SampleEscherichia coli
Sample nameE. coli strain BL21
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID2185
Identified moleculeprotein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34276573   
OrganismEscherichia coli
Experiment descriptionDifferential Packaging Into Outer Membrane Vesicles Upon Oxidative Stress Reveals a General Mechanism for Cargo Selectivity
AuthorsOrench-Rivera N, Kuehn MJ.
Journal name Front Microbiol
Publication year2021
SampleEscherichia coli
Sample nameE. coli strain BL21
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID2232
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain DH5α - 9h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
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5
Experiment ID2233
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain DH5α - 12h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
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6
Experiment ID2234
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain DH5α - 18h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID2235
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain DH5α - 24h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID2236
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain BL21 (ClearColi) - 18h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID2237
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain BL21 (ClearColi) - 21h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID2238
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain BL21 (ClearColi) - 24h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID2239
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain BL21 (ClearColi) - 27h culture
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID2244
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 6
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

13
Experiment ID2245
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 7
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

14
Experiment ID2246
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 8
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

15
Experiment ID2247
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 9
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

16
Experiment ID2248
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 10
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

17
Experiment ID2249
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 11
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

18
Experiment ID2250
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 12
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

19
Experiment ID2251
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

20
Experiment ID2253
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

21
Experiment ID2254
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

22
Experiment ID2255
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

23
Experiment ID2256
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

24
Experiment ID2257
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

25
Experiment ID2219
Identified moleculeProtein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 32854612   
OrganismEscherichia coli
Experiment descriptionProbiotic Escherichia coli Nissle 1917-derived outer membrane vesicles enhance immunomodulation and antimicrobial activity in RAW264.7 macrophages
AuthorsHu R, Lin H, Li J, Zhao Y, Wang M, Sun X, Min Y, Gao Y, Yang M.
Journal name BMC Microbiol
Publication year2020
SampleEscherichia coli
Sample nameE. coli strain Nissle 1917
Isolation/purification methodsCentrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density1.127-1.175 g/mL, 1.271-1.295 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
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